Primary Kupffer Cells
Primary Kupffer cells (PKC) are specialized macrophage residents in the liver, lining the walls of the sinusoids. Primary Kupffer cells are part of the immune system and can be activated to produce inflammatory cytokines, growth factors, and reactive oxygen species.
Furthermore, PKC are part of the first line of defense against bacteria and toxins from the gut and perform an efficient uptake of senescence red blood cells in the blood vessel for the transfer of iron to hepatocytes. In addition, powerful lysosomal enzymes are found in Kupffer cells and as a result, many substances that are ingested and partially degraded by them are passed on to hepatocytes, thus performing key functions in the liver.
Primary Kupffer Cells are a valuable research tool for studying liver resident macrophages in fields such as immunology, drug metabolism, and toxicity testing. We offer a variety of Primary Kupffer Cells isolated from healthy human liver tissue donors and conduct comprehensive characterization to ensure optimal performance in vitro. Our rigorous two-phase approach includes determining cell number per vial, viability, and cell seeding density, as well as testing for specific markers using flow cytometry.
Primary Kupffer Cells provides researchers with a high-quality tool to study the role of these important liver resident macrophages in various fields such as immunology, drug metabolism, and toxicity testing. BeCytes Kupffer Cells are isolated from healthy human liver tissue donors and undergo rigorous quality control testing to ensure optimal performance in vitro.
Our product catalog includes a variety of Primary Kupffer Cells suitable for different research applications. We offer Primary Kupffer Cells from donors of different ages and genders, to suit your specific research needs.
At our facility, we conduct comprehensive characterization of our Primary Kupffer Cells to ensure their quality and suitability for various research applications.
Our extensive characterization process is a crucial part of our commitment to providing you with high-quality research tools. Through our rigorous two-phase approach, we not only determine cell number per vial, viability, and cell seeding density but also test for specific markers using flow cytometry to provide accurate and reliable results for their research applications.
Kupffer cells characterization
Basic Characterization
- Cell number and viability after thawing
- Morphological analysis of cells over time
Additional Characterization
- The principal markers present in the Kupffer Cells are used to determine their expression and percentage.
- LPS induction assays
Common Uses
Useful tool to study
- Disease progression
- Pro-inflammatory Metabolism
- Anti-inflammatory balance
In vitro co-cultures with Kupffer Cells can also be used for:
- Toxicity tests
- Drug screening
Primary Kupffer Cells are produced under rigorous QC standards and supplied with:
- Demographic and clinical donor profile
- Viability and morphology assessment
- Specific COA with culture and maintenance protocols
- Kupffer cells media
Shipping
We avoid shipments arriving at weekends or holidays.
We store and ship all cell products at the most favorable temperature range for the product and we monitor the safety and integrity of shipped products proactively.
All of our products are delivered in compliance of the IATA Dangerous Goods Regulations.
Shipping conditions: Dry ice (-80ºC)/ LN (-196ºC)
Packaging
Packaging and shipping fresh products to preserve its quality when transporting, to ensure that all primary cell products are protected with integrity and viability.
Leak-proof packaging materials that meet the general requirements of carriers and customs authorities, where applicable usually ship at room temperature.
Obtain personalized advice from Cytes Scientific and Sales experts
At Cytes Biotechnologies we will personally advise you to obtain the liver cells that best suit the conditions of your project.
From the moment you contact us, our experts will follow the following steps
- Detailed study of the requirements described on the form
- Personalized advice according to the needs of each client
- Permanent contact throughout the process to ensure the best results
Fill in the following form and we will contact you with an answer to your request.
