Primary Fresh Human Hepatocytes

Fresh human hepatocytes

Fresh Primary Human Hepatocytes (HuHEF), derived from liver tissue unsuitable for transplantation, are primary liver cells with high relevance for in vitro studies.

These hepatocytes retain essential characteristics of liver cells found in the human body, such as metabolic activities, drug-metabolizing enzymes, and transporters.

Fresh human hepatocytes for research purposes are obtained and isolated from a wide diversity of human donors.


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The liver is the largest gland in the body, and 70–85% of liver volume is occupied by hepatocytes. Hepatocytes are specialized epithelial cells that exhibit the highly polarized architecture and organization of the cytoplasm and plasma membrane, to fulfill the multitude of tasks they are required to perform. These cells play pivotal roles in metabolism, detoxification and protein synthesis. Specifically, primary human hepatocytes are isolated directly from the liver, and they have become the «gold standard» for evaluating hepatic metabolism and the toxicity of drugs and other xenobiotics in vitro.
Primary human hepatocytes are considered the gold standard for in vitro hepatic studies, including ADME/DMPK. Although primary human hepatocytes are not proliferative cells in an in vitro culture, these cells retain most, if not all, of their original biochemical, molecular signaling pathways, along with phenotypic gene expression profiles. Conversely, immortalized cells have undergone genetic modifications that lead to uncoupled growth characteristics, and they cannot fully replicate the phenotypes found in primary hepatocytes.
Hepatocytes are determined to be either plateable or suspension based on the confluency or the percentage of a culture vessel, which is covered after plating the cells. To be considered plateable, our internal quality control department has established that the cells must create a monolayer covering around 70% of the plate’s total surface area; if the cells do not accomplish the mentioned criteria, they are considered suspension hepatocytes.Plateable hepatocytes are advantageous because they can be used for long-term culturing applications, and can be utilized in many drug metabolism and pharmacokinetics-related preclinical studies, CYP induction assays, inhibitions assays, host-pathogen interactions experiments, as well as 3D hepatic models.Suspension hepatocytes are used for short-term experiments, between 2 to 8 hours depending on how rapidly the viability decreases over time, and their applications are related to studies for compound stability, metabolite formation, inhibitions studies, uptake.
Donor demographics including age, gender, race, BMI, smoking habit, alcohol use, and drug use are typically made available to researchers. The results of the pathological and serological tests conducted prior to liver collection are also available. Other information, including drugs administered during hospitalization, medical prescriptions used by the patient, and other relevant medical histories may also be provided.
We do not recommend splitting the primary hepatocytes, since these cells are not proliferative, and they are very sensitive.
We source liver tissue from planned surgical resections on living donors, after the patient has been informed that the resected tissue will be used for research purposes and their informed consent has been obtained to maintain ethical standards. Cytes collaborates with an extensive network of hospitals to obtain tissues, abiding by all appropriate legal channels.
We perform regular liver isolations, which allow us to ship freshly-isolated liver cells in suspensions or plated formation. To be notified about freshly-isolated human liver cells ahead of time you must contact us at or fill out the form to receive hepatic donor alerts at Our system notifies the interested parties of all potential donors.
Cytes Biotechnologies provides all thawing, plating, and maintenance mediums, all necessary to support every step of your research process.
An accurate cell count is vital to the success of your experiment, because both under-seeding and over-seeding influence the quality of the sample. We encourage the use of consistent techniques to measure cell viability and to ensure accuracy. Also, in our CoA we include the number of cells and the viability with the respective standard deviation for each parameter, so any client can compare their cell count to ours.
Cytes have the capability to ship the cells in a dry liquid nitrogen vapor transporter or on dry ice, depending on the customer’s destination to ensure that the cells arrive in perfect condition. We recommend immediately placing the cryogenic hepatocytes vials into the gas phase of the liquid nitrogen tank when the cells arrive to the facilities.
Cryopreserved 3D spheroid qualified human hepatocytes are primary hepatocytes, which have the ability to self-assemble into a 3D spheroid by using our protocol, ultra-low attachment plates and our 3D plating and maintenance medium.
Fresh hepatocytes are the cells obtained and distributed directly after liver digestion. Our fresh samples are available in both suspension and plated forms. Ideally, fresh samples are immediately used upon arrival at the research laboratory.Cryopreserved hepatocytes are more commonly used in research, as being cryopreserved grants the flexibility to organize your experiments. Cytes can provide plated and cryopreserved hepatocytes, this last option also available in both suspension and plateable formats.But regardless of your preference for fresh or cryopreserved hepatocytes, we will work with you to provide the best possible sample to meet your research needs.

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At Cytes Biotechnologies we will personally advise you to obtain the liver cells that best suit the conditions of your project.

From the moment you contact us, our experts will follow the following steps

  1. Detailed study of the requirements described on the form
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