Isolation of non-parenchymal cells (NPCs) provides liver models that mimic the microenvironment and microanatomy of the human liver for the study of liver functions. Moreover, these models show organotypic interactions between hepatocytes and non-parenchymal cells through direct contact or paracrine signalling. Therefore, these models are able to recreate the physiological environment in vivo and this is a fundamental requirement to obtain a specific predictive response for the study of liver physiology/pathology or to perform new drug screenings and safety analyses with a high predictive value.