Pathological ​Hepatocytes (MASH, Esteatotic, Fibrotic…)

Nash Hepatocytes

BeCytes Biotechnology is dedicated to providing MASLD hepatocytes, sourced directly from diagnosed patients, to advance research and development in this growing epidemic.

MASLD hepatocytes are primary human hepatocytes exposed to conditions that mimic metabolic dysfunction-associated steatotic liver disease (MASLD) and its more advanced form, metabolic dysfunction-associated steatohepatitis (MASH).

These cells offer a biologically relevant model for studying this increasingly prevalent liver disease.

Ref. Product
HuHECSM – MASH Suspension – Metabolism Certified and MASH Diagnosed

Hepatocytes from MASH donor:

MASLD Hepatocytes Characterization
  • Cell number and viability post-thawing
  • Viability over time

What is MASLD?

Metabolic dysfunction-associated steatohepatitis (MASH) is the advanced form of metabolic dysfunction-associated steatotic liver disease (MASLD). It is characterized by excessive fat accumulation in liver cells, leading to inflammation, hepatocyte ballooning, and hepatic fibrosis. Patients with MASH are at high risk of developing cirrhosis, hepatocellular carcinoma (HCC), cardiovascular diseases, and chronic kidney disease.

With MASLD prevalence expected to rise significantly, understanding the pathophysiological mechanisms of this disease is crucial for early detection, prevention, and drug discovery.

MASLD hepatocytes from diagnosed donors provide a valuable tool for studying liver disease progression and potential treatments. In the near future, MASH is anticipated to become one of the leading causes of liver transplantation.

BeCytes Biotechnologies MASLD Hepatocytes Documentation

A certificate of analysis is provided, including data related to the donor’s medical record, as well as the characterization of MASLD hepatocytes, such as the number of cells per vial and post-thaw viability over time. Additionally, MASLD hepatocytes are certified for in-vitro enzyme expression levels.

Features and Benefits

BeCytes Biotechnologies’ Mash Hepatocytes are isolated from single donors and processed the same day to ensure their best performance.

Shipping
We avoid shipments arriving at weekends or holidays.
We store and ship all cell products at the most favorable temperature range for the product, and we monitor the safety and integrity of shipped products proactively.
All of our products are delivered in compliance of the IATA Dangerous Goods Regulations.
Packaging

Packaging and shipping fresh products to preserve its quality when transporting, to ensure that all primary cell products are protected with integrity and viability.

Leak-proof packaging materials that meet the general requirements of carriers and customs authorities, where applicable usually ship at room temperature.

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FAQ

The liver is the largest gland in the body, and 70–85% of liver volume is occupied by hepatocytes. Hepatocytes are specialized epithelial cells that exhibit the highly polarized architecture and organization of the cytoplasm and plasma membrane, to fulfill the multitude of tasks they are required to perform. These cells play pivotal roles in metabolism, detoxification and protein synthesis. Specifically, primary human hepatocytes are isolated directly from the liver, and they have become the “gold standard” for evaluating hepatic metabolism and the toxicity of drugs and other xenobiotics in vitro.

Hepatocytes are determined to be either plateable or suspension based on the confluency or the percentage of a culture vessel, which is covered after plating the cells. To be considered plateable, our internal quality control department has established that the cells must create a monolayer covering around 70% of the plate’s total surface area; if the cells do not accomplish the mentioned criteria, they are considered suspension hepatocytes.

Plateable hepatocytes are advantageous because they can be used for long-term culturing applications, and can be utilized in many drug metabolism and pharmacokinetics-related preclinical studies, CYP induction assays, inhibitions assays, host-pathogen interactions experiments, as well as 3D hepatic models.

Suspension hepatocytes are used for short-term experiments, between 2 to 8 hours depending on how rapidly the viability decreases over time, and their applications are related to studies for compound stability, metabolite formation, inhibitions studies, uptake.

Fresh hepatocytes are the cells obtained and distributed directly after liver digestion. Our fresh samples are available in both suspension and plated forms. Ideally, fresh samples are immediately used upon arrival at the research laboratory.

Cryopreserved hepatocytes are more commonly used in research, as being cryopreserved grants the flexibility to organize your experiments. Cytes can provide plated and cryopreserved hepatocytes, this last option also available in both suspension and plateable formats.

But regardless of your preference for fresh or cryopreserved hepatocytes, we will work with you to provide the best possible sample to meet your research needs.

Donor demographics including age, gender, race, BMI, smoking habit, alcohol use, and drug use are typically made available to researchers. The results of the pathological and serological tests conducted prior to liver collection are also available. Other information, including drugs administered during hospitalization, medical prescriptions used by the patient, and other relevant medical histories may also be provided.

All Cytes products come with a certificate of analysis (CoA), including data related to the donor’s medical record and histological analysis from the tissue and before isolation. It also includes the number of hepatocytes per vial and its viability post-thawing; as well as the optimal cell seeding concentration in different culture plates, number of days in cultures, and morphology pictures. Information about the enzyme expression levels, induction studies, and spheroid formation of the hepatocytes is also available.

We do not recommend splitting the primary hepatocytes, since these cells are not proliferative, and they are very sensitive.

We source liver tissue from planned surgical resections on living donors, after the patient has been informed that the resected tissue will be used for research purposes and their informed consent has been obtained to maintain ethical standards. Cytes collaborates with an extensive network of hospitals to obtain tissues, abiding by all appropriate legal channels.

Cytes Biotechnologies provides all thawing, plating, and maintenance mediums, all necessary to support every step of your research process.

An accurate cell count is vital to the success of your experiment, because both under-seeding and over-seeding influence the quality of the sample. We encourage the use of consistent techniques to measure cell viability and to ensure accuracy.

Also, in our CoA we include the number of cells and the viability with the respective standard deviation for each parameter, so any client can compare their cell count to ours.

Cryopreserved hepatocytes can last several years if properly maintained. This makes them ideal for research that require the use of the same cells over several months.

Cytes have the capability to ship the cells in a dry liquid nitrogen vapor transporter or on dry ice, depending on the customer’s destination to ensure that the cells arrive in perfect condition. We recommend immediately placing the cryogenic hepatocytes vials into the gas phase of the liquid nitrogen tank when the cells arrive to the facilities.

Cryopreserved 3D spheroid qualified human hepatocytes are primary hepatocytes, which have the ability to self-assemble into a 3D spheroid by using our protocol, ultra-low attachment plates and our 3D plating and maintenance medium.

Cytes have the capability to ship the cells in a dry liquid nitrogen vapor transporter or on dry ice, depending on the customer’s destination to ensure that the cells arrive in perfect condition. We recommend immediately placing the cryogenic hepatocytes vials into the gas phase of the liquid nitrogen tank when the cells arrive to the facilities.

Cryopreserved 3D spheroid qualified human hepatocytes are primary hepatocytes, which have the ability to self-assemble into a 3D spheroid by using our protocol, ultra-low attachment plates and our 3D plating and maintenance medium.

1. Do not leave hepatocytes floating in the cryopreservation medium for too long if you don’t want to lose viability:

· After removing the cryopreserved hepatocytes from the liquid nitrogen, immediately place them at 37ºC in a water bath until the cell suspension is partially thawed.

· Quick transfer hepatocytes into a preheated thawing medium.

2. Seed the correct cell density to obtain a confluent monolayer of hepatocytes:

· Evenly distribute the cells throughout by manually shaking the plate in a T-shape and checking it under a microscope.

· Incubate the cells at 37ºC and move the plate in a T-shape every 15 minutes for the first hour.

· For 96-well plates, initially dose 60uL of a pre-heated plating medium and then add the cells to the well.

Cytes do not recommend exposing primary hepatocytes to repeated freezing and thawing cycles, due to the additional stress and damage imposed on the cells and their subcellular components.

Obtain personalized advice from Cytes Scientific and Sales experts

At Cytes Biotechnologies we will personally advise you to obtain the liver cells that best suit the conditions of your project.

From the moment you contact us, our experts will follow the following steps

  1. Detailed study of the requirements described on the form
  2. Personalized advice according to the needs of each client
  3. Permanent contact throughout the process to ensure the best results

Fill in the following form and we will contact you with an answer to your request.