NASH ​Hepatocytes

Nash Hepatocytes

BeCytes Biotechnology is dedicated to providing NASH hepatocytes, which have recently been renamed MASLD, directly from diagnosed patients to advance the development of research into this growing epidemic.

MASLD hepatocytes formerly known as NASH are primary human hepatocytes that have been exposed to conditions that mimic non-alcoholic fatty liver disease (NAFLD) and non-alcoholic steatohepatitis (NASH).

These cells (MASLD hepatocytes formerly known as NASH) offer a direct link to the study of this increasingly prevalent liver disease.

Ref. Product
HuHECSM – NASH Suspension – Metabolism Certified and NASH Diagnosed
Hepatocytes from NASH donor:
NASH Hepatocytes Charaterization
  • Cell number and viability post-thawing
  • Viability over time

 

What is NASH?

Non-Alcoholic Steatohepatitis (NASH) is the most advanced form of non-alcoholic fatty liver disease (NAFLD) characterized by an accumulation of fat in the liver cells that causes inflammation, ballooning of the liver cells, and hepatic fibrosis. Patients suffering from NASH often develop cirrhosis followed by hepatocellular carcinoma (#HCC), a life-threatening disease. Similarly, those affected with #NASH have been linked to an increased risk of developing cardiovascular diseases and chronic kidney disease. With prevalence expected to increase by 63% between 2015 and 2030, identifying the pathophysiological features of NASH is critical for disease prevention, early detection, and drug discovery.

NASH Hepatocytes from diagnosed donors will help us better understand the causes and treatments of liver diseases such as cirrhosis and liver cancer. In the near future, NASH is expected to be one of the most common reasons for liver transplantation.

BeCytes Biotechnologies NASH Hepatocytes Documentation

Certificate of analysis is provided with data related to the donor’s medical record, as well as NASH hepatocyte characterization including the number of cells per vial and viability after thawing in a time frame. Also, NASH Hepatocytes are certified for in-vitro like enzyme expression levels.

Features and Benefits

BeCytes Biotechnologies’ Nash Hepatocytes are isolated from single donors and processed the same day to ensure their best performance.

Shipping
We avoid shipments arriving at weekends or holidays.
We store and ship all cell products at the most favorable temperature range for the product, and we monitor the safety and integrity of shipped products proactively.
All of our products are delivered in compliance of the IATA Dangerous Goods Regulations.
Packaging

Packaging and shipping fresh products to preserve its quality when transporting, to ensure that all primary cell products are protected with integrity and viability.

Leak-proof packaging materials that meet the general requirements of carriers and customs authorities, where applicable usually ship at room temperature.

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FAQ

The liver is the largest gland in the body, and 70–85% of liver volume is occupied by hepatocytes. Hepatocytes are specialized epithelial cells that exhibit the highly polarized architecture and organization of the cytoplasm and plasma membrane, to fulfill the multitude of tasks they are required to perform. These cells play pivotal roles in metabolism, detoxification and protein synthesis. Specifically, primary human hepatocytes are isolated directly from the liver, and they have become the “gold standard” for evaluating hepatic metabolism and the toxicity of drugs and other xenobiotics in vitro.

Hepatocytes are determined to be either plateable or suspension based on the confluency or the percentage of a culture vessel, which is covered after plating the cells. To be considered plateable, our internal quality control department has established that the cells must create a monolayer covering around 70% of the plate’s total surface area; if the cells do not accomplish the mentioned criteria, they are considered suspension hepatocytes.

Plateable hepatocytes are advantageous because they can be used for long-term culturing applications, and can be utilized in many drug metabolism and pharmacokinetics-related preclinical studies, CYP induction assays, inhibitions assays, host-pathogen interactions experiments, as well as 3D hepatic models.

Suspension hepatocytes are used for short-term experiments, between 2 to 8 hours depending on how rapidly the viability decreases over time, and their applications are related to studies for compound stability, metabolite formation, inhibitions studies, uptake.

Fresh hepatocytes are the cells obtained and distributed directly after liver digestion. Our fresh samples are available in both suspension and plated forms. Ideally, fresh samples are immediately used upon arrival at the research laboratory.

Cryopreserved hepatocytes are more commonly used in research, as being cryopreserved grants the flexibility to organize your experiments. Cytes can provide plated and cryopreserved hepatocytes, this last option also available in both suspension and plateable formats.

But regardless of your preference for fresh or cryopreserved hepatocytes, we will work with you to provide the best possible sample to meet your research needs.

Donor demographics including age, gender, race, BMI, smoking habit, alcohol use, and drug use are typically made available to researchers. The results of the pathological and serological tests conducted prior to liver collection are also available. Other information, including drugs administered during hospitalization, medical prescriptions used by the patient, and other relevant medical histories may also be provided.

All Cytes products come with a certificate of analysis (CoA), including data related to the donor’s medical record and histological analysis from the tissue and before isolation. It also includes the number of hepatocytes per vial and its viability post-thawing; as well as the optimal cell seeding concentration in different culture plates, number of days in cultures, and morphology pictures. Information about the enzyme expression levels, induction studies, and spheroid formation of the hepatocytes is also available.

We do not recommend splitting the primary hepatocytes, since these cells are not proliferative, and they are very sensitive.

We source liver tissue from planned surgical resections on living donors, after the patient has been informed that the resected tissue will be used for research purposes and their informed consent has been obtained to maintain ethical standards. Cytes collaborates with an extensive network of hospitals to obtain tissues, abiding by all appropriate legal channels.

Cytes Biotechnologies provides all thawing, plating, and maintenance mediums, all necessary to support every step of your research process.

An accurate cell count is vital to the success of your experiment, because both under-seeding and over-seeding influence the quality of the sample. We encourage the use of consistent techniques to measure cell viability and to ensure accuracy.

Also, in our CoA we include the number of cells and the viability with the respective standard deviation for each parameter, so any client can compare their cell count to ours.

Cryopreserved hepatocytes can last several years if properly maintained. This makes them ideal for research that require the use of the same cells over several months.

Cytes have the capability to ship the cells in a dry liquid nitrogen vapor transporter or on dry ice, depending on the customer’s destination to ensure that the cells arrive in perfect condition. We recommend immediately placing the cryogenic hepatocytes vials into the gas phase of the liquid nitrogen tank when the cells arrive to the facilities.

Cryopreserved 3D spheroid qualified human hepatocytes are primary hepatocytes, which have the ability to self-assemble into a 3D spheroid by using our protocol, ultra-low attachment plates and our 3D plating and maintenance medium.

Cytes have the capability to ship the cells in a dry liquid nitrogen vapor transporter or on dry ice, depending on the customer’s destination to ensure that the cells arrive in perfect condition. We recommend immediately placing the cryogenic hepatocytes vials into the gas phase of the liquid nitrogen tank when the cells arrive to the facilities.

Cryopreserved 3D spheroid qualified human hepatocytes are primary hepatocytes, which have the ability to self-assemble into a 3D spheroid by using our protocol, ultra-low attachment plates and our 3D plating and maintenance medium.

1. Do not leave hepatocytes floating in the cryopreservation medium for too long if you don’t want to lose viability:

· After removing the cryopreserved hepatocytes from the liquid nitrogen, immediately place them at 37ºC in a water bath until the cell suspension is partially thawed.

· Quick transfer hepatocytes into a preheated thawing medium.

2. Seed the correct cell density to obtain a confluent monolayer of hepatocytes:

· Evenly distribute the cells throughout by manually shaking the plate in a T-shape and checking it under a microscope.

· Incubate the cells at 37ºC and move the plate in a T-shape every 15 minutes for the first hour.

· For 96-well plates, initially dose 60uL of a pre-heated plating medium and then add the cells to the well.

Cytes do not recommend exposing primary hepatocytes to repeated freezing and thawing cycles, due to the additional stress and damage imposed on the cells and their subcellular components.

Obtain personalized advice from Cytes Scientific and Sales experts

At Cytes Biotechnologies we will personally advise you to obtain the liver cells that best suit the conditions of your project.

From the moment you contact us, our experts will follow the following steps

  1. Detailed study of the requirements described on the form
  2. Personalized advice according to the needs of each client
  3. Permanent contact throughout the process to ensure the best results

Fill in the following form and we will contact you with an answer to your request.